To develop a promising antibacterial agent for wound-care dressing, a series of silver nanoparticles (AgNPs) and eggshell membrane (ESM) composites (AgNPs/ESM) were prepared. AgNPs were prepared using a chemical reduction method and their characteristics were determined. Various pH and processing time combinations were tested to find the optimal conditions for preparing AgNPs/ESM composites. To obtain the optimal nontoxic-level silver release, the AgNPs stock solution was diluted to 2 times, 4 times, 6 times, 8 times and 10 times with water and the concentration of silver released by the composites was also tested. All the prepared composites showed antibacterial activity, but the activity was the strongest when the stock AgNPs solution was diluted to four times (a concentration of silver was 2.41 mg/L). The addition of AgNPs changed the ESM from hydrophobic to hydrophilic by lowering the water contact angles from 105° to 75°, which is important for the wound-healing process. And the AgNPs/ESM composites had a higher surface area (159.08 m/g) than the natural ESM (24.32 m/g) and a suitable pore size (10.92 nm) as well, endowing with better absorption and antibacterial abilities. These findings suggested that the AgNPs/ESM composites are promising candidates for the development of antimicrobial agent for biomedical devices and therapeutic applications, such as wound-healing agent.