Background and Aim: Recently, researchers have increasingly turned to study the relation between stuttering and syntactic complexity. This study investigates the effect of syntactic complexity on the amount of speech dysfluency in stuttering Persian-speaking children and adults in conversational speech. The obtained results can pave the way to a better understanding of stuttering in children and adults, and finding more appropriate treatments. Methods: In this cross-sectional study, the participants were 15 stuttering adult Persian-speakers, older than 15 years, and 15 stuttering child Persian-speakers of 4-6 years of age. In this study, first a 30 minute sample of the spontaneous speech of the participants was provided. Then the utterances of each person were studied in respect to the amount of dysfluency and syntactic complexity. The obtained information was analyzed using paired samples t-test. Results: In both groups of stuttering children and adults, there was a significant difference between the amount of dysfluency of simple and complex sentences (p<0.05). Conclusion: The results of this study showed that an increase in syntactic complexity in conversational speech, increased the amount of dysfluency in stuttering children and adults. Moreover, as a result of increase of syntactic complexity, dysfluency had a greater increase in stuttering children than stuttering adults.