Use of paraffin-embedded tissue for identification of Saccharomyces cerevisiae in a baker's lung nodule by fungal PCR and nucleotide sequencing
Abstract
A 40-year-old healthy male employed in a bakery presented with a single lung nodule and underwent investigations to rule out pulmonary carcinoma. Biopsy was positive for yeast cells, which did not match common fungal pathogens. PCR assay of paraffin-embedded tissue and nucleotide sequencing with rib …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
humans
pubmed abstract
nih
national institutes of health
national library of medicine
adult
male
case reports
DNA
Sequence Analysis
Polymerase Chain Reaction / methods*
Base Sequence
Molecular Sequence Data
occupational exposure
lung / microbiology*
saccharomyces cerevisiae / isolation & purification*
pmid:15184487
pmc427872
doi:10.1128/jcm.42.6.2840-2842.2004
ping ren
sundara sridhar
vishnu chaturvedi
paraffin embedding
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273422
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