Vir typing: a long-PCR typing method for group A streptococci
Abstract
We have developed a new procedure (Vir typing) for typing Streptococcus pyogenes, by amplifying the entire 5- to 7-kb variable vir regulon by long PCR. The amplified DNA is then cleaved with HaeIII and visualized by ethidium bromide fluorescence after agarose gel electrophoresis. A simple procedure …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
genetic
humans
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
DNA
sensitivity and specificity
Polymerase Chain Reaction / methods*
Base Sequence
Molecular Sequence Data
Polymorphism
bacterial proteins / genetics*
streptococcus pyogenes / isolation & purification
bacterial / genetics
dna primers
streptococcal infections / microbiology*
streptococcus pyogenes / classification*
bacterial / isolation & purification
streptococcus pyogenes / genetics
streptococcal infections / transmission
restriction mapping
k s sriprakash
virulence factors*
pmid:7580916
doi:10.1101/gr.4.5.288
d gardiner
j hartas
endodeoxyribonucleases / genetics
regulon
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272766
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