Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays
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Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays

Drmanac R;Sparks AB;Callow MJ;Halpern AL;Burns NL;Kermani BG;Carnevali P;Nazarenko I;Nilsen GB;Yeung G;Dahl F;Fernandez A;Staker B;Pant KP;Baccash J;Borcherding AP;Brownley A;Cedeno R;Chen L;Chernikoff D;Cheung A;Chirita R;Curson B;Ebert JC;Hacker CR;Hartlage R;Hauser B;Huang S;Jiang Y;Karpinchyk V;Koenig M;Kong C;Landers T;Le C;Liu J;McBride CE;Morenzoni M;Morey RE;Mutch K;Perazich H;Perry K;Peters BA;Peterson J;Pethiyagoda CL;Pothuraju K;Richter C;Rosenbaum AM;Roy S;Shafto J;Sharanhovich U;Shannon KW;Sheppy CG;Sun M;Thakuria JV;Tran A;Vu D;Zaranek AW;Wu X;Drmanac S;Oliphant AR;Banyai WC;Martin B;Ballinger DG;Church GM;Reid CA;;
Science (New York, N.Y.) 2010 Vol. 327 pp. -
299
r2010sciencehuman

Abstract

Genome sequencing of large numbers of individuals promises to advance the understanding, treatment, and prevention of human diseases, among other applications. We describe a genome sequencing platform that achieves efficient imaging and low reagent consumption with combinatorial probe anchor ligatio …

Keywords

software databases National Center for Biotechnology Information NCBI NLM MEDLINE nanotechnology humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't male N.I.H. Extramural Sequence Analysis genome Base Sequence Genotype Polymorphism DNA / methods* computational biology nucleic acid costs and cost analysis nucleic acid amplification techniques single nucleotide human* dna / economics dna / standards haplotypes dna / genetics human genome project pmid:19892942 doi:10.1126/science.1181498 radoje drmanac andrew b sparks clifford a reid dna / chemistry* genomic library microarray analysis* nanostructures dna / instrumentation

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http://www.ncbi.nlm.nih.gov/pubmed/19892942

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