The mechanism and regulation of deadenylation: identification and characterization of Xenopus PARN
Abstract
In Xenopus oocytes, the deadenylation of a specific class of maternal mRNAs results in their translational repression. Here we report the purification, characterization, and molecular cloning of the Xenopus poly(A) ribonuclease (xPARN). xPARN copurifies with two polypeptides of 62 kDa and 74 kDa, an …
Keywords
National Center for Biotechnology Information
NCBI
NLM
MEDLINE
Molecular
animals
pubmed abstract
nih
national institutes of health
national library of medicine
research support
non-u.s. gov't
u.s. gov't
non-p.h.s.
amino acid sequence
P.H.S.
DNA
Molecular Sequence Data
complementary
sequence homology
amino acid
electrophoresis
cloning
chromatography
substrate specificity
polyacrylamide gel
xenopus
pmid:11424938
pmc1370141
doi:10.1017/s1355838201010020
p r copeland
m wormington
ion exchange
exoribonucleases / chemistry*
exoribonucleases / genetics
exoribonucleases / isolation & purification
exoribonucleases / metabolism
poly a / metabolism*
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266979
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