Morels are wild edible ascomycetous mushrooms that are highly prized because of their medicinal and nutritional qualities. Ligninolytic enzymes are considered as one of the most important enzymes in fungi due to their involvement in fruiting body formation during artificial cultivation on different substrates. In the life cycle of morels, sclerotia are the intermediate stage to form a fruiting body from mycelia. We have studied the production of ligninolytic enzymes by Morchella crassipes MR8 growing on different substrates and during sclerotia formation. This fungus is able to produce ligninolytic enzymes such as laccase (Lac), lignin peroxidase (LiP), and manganese-dependent peroxidase (MnP) when grown on different substrates. Maximum Lac activity was observed when grown in wheat grains whereas maximum activities of MnP and LiP were observed when grown in rice straw. Laccase enzyme was produced in high titers during sclerotia formation and maturation when grown in combinations of soil and substrates. A large number of sclerotia was observed in soil and wheat grains, along with high titers of laccase. Cellulase activity was observed to be constant during sclerotia formation and maturation. The present study results suggest that laccase enzyme might play an important role in sclerotia formation in morels.