The continuous development of microgrid’s technology creates favorable conditions for the access of distributed energy. Firstly, in order to consider the interests of the demand side and the power side, this paper presents a dual-layer optimal dispatching model of microgrid based on demand response. The objective of the first-layer optimization is to obtain the maximum load satisfaction and to optimize the load curve. The objective of the second-layer optimization is to make the microgrid system economical and environmentally friendly and to optimize the power utilization ratio. And a microsource control strategy based on the isolated microgrid is proposed, which can optimize the operation state of battery and improve the economy of the system. Finally, the Nondominated Sorting Genetic Algorithm-II (NSGA-II) is adopted to solve the optimal scheduling problem of the isolated microgrid. The simulation results indicate that the microsource scheduling strategy proposed in this paper can improve the operation economy and environmental conservation of the system. It can improve the reliability of microgrid power supply and reduce energy waste.