In the previous study, reports of human immunodeficiency virus (HIV)-associated multiple tissue dysfunction syndromes are limit. Now, multiple tissue lesions of one boy with AIDS were analyzed using X-ray, computed tomography (CT) and magnetic resonance imaging (MRI). Results showed that obvious brain atrophy and several focus were detected by CT and MRI. Lung lymphoid inflammation and pulmonary lymphoplasia were observed by CT. A swelling with a slight low density in hepatic anterior region of right lobe and a large soft tissue neoplasm in right abdomen were obtained by CT. An obvious damage of intestinal duct and intestinal lymphomas were respectively observed by molybdenum contrast image and CT. X-ray showed a low density of thoracic bone and iliac bone, and irregular shapes of bone joint and epiphysis. Characterizations of HIV-associated multiple organ dysfunction syndromes in the case are benefit to understand features of children with acquired immune deficiency syndrome.