A total of 107 spider species from 15 families were recorded in the herbaceous vegetation of the steppe ecosystems of Ukraine and the Central Chernozem region of Russia. Araneidae, Thomisidae, Salticidae and Theridiidae were the most species-rich. The species composition depended on the steppe type; adjacent forest habitats influenced steppe fauna in the forest-steppe and northern part of the steppe natural zone. The number of generalist, forest and wetland dwelling species in the steppe vegetation showed a tendency to decrease towards the south. Dominance of herb-dwelling spiders was specific to each steppe type; no single species was found to predominate in all the steppe habitats.