BACKGROUND: The school environment is considered suitable for promoting physical activity in children, which influences their attitude not only to physical activity but also to themselves, classmates, and teachers. Besides the family, the school environment is a significant scene for the development of life values, which includes a positive children's attitude to regular physical activity. AIMS: The objective of the study is to use a school-level questionnaire to analyse spatial and organizational conditions for physical activity and schoolbased physical education in primary and secondary schools in the Czech Republic (CR). METHODS: To investigate the conditions for physical activity and physical education in schools in the CR, a selection sample of 92 educational institutions (84 primary schools and 8 multi-year grammar schools) were used. Research data were collected in the first half of 2012. Statistical data processing was performed by means of descriptive statistics and graphic illustration from the questionnaire. RESULTS: Apart from physical education lessons, schools provide additional forms of after-school physical activity. As much as 29.4% of schools provide afterschool physical activity, while physically active breaks are promoted by 26.8% of schools. More than 80% of schools have sufficient equipment for the delivery of physical activity and use it on a daily basis. Only in 17% of schools students have free access to this equipment during breaks and free lessons. CONCLUSIONS: Most schools in the CR have sufficient space for the delivery of children's physical activity. The most frequent physical activity during school lessons is physical education, which is delivered in two lessons a week. Promotion of physical activity during breaks and free lessons appears significant in terms of overall physical activity levels in children.