Two Si-based micro pulsating heat pipes (µPHPs) charged using HFE-7100 were either horizontally or vertically oriented and were tested using several heating powers. The width of each channel was 0.8 mm in one µPHP containing uniform channels, and the channel width was 1.0 mm or 0.6 mm in the other µPHP, which did not contain uniform channels. The depth of each channel was 0.25 mm. The overall size of each µPHP was 60 × 10 × 1.25 mm. Visual observation and temperature measurement of the µPHPs under various conditions were performed and the results were analyzed. The results indicated that when the µPHPs were operated horizontally at a heating power ranging from 1 to 7 W, the pulsating two-phase flow in the channels of the µPHPs could not begin, except when the µPHP containing nonuniform channels was tested at a heating power of 7 W. With a heating power less than 5 W, the frequency of the sine-like oscillating displacement of the vapor slug increased and the displacement of the vapor slug reduced in either vertically oriented μPHP, as the heating power increased With a heating power higher than 5 W, periodic “start-stop” behaviors were observed in the vertical μPHP containing nonuniform channels.