improvement of biotechnological xylitol production by glucose during cultive of candida guilliermondii in sugarcane bagasse hydrolysate

improvement of biotechnological xylitol production by glucose during cultive of candida guilliermondii in sugarcane bagasse hydrolysate

;Débora Danielle Virgínio da Silva;Ismael Maciel de Mancilha;Silvio Silvério da Silva;Maria das Graças de Almeida Felipe
current drug delivery 2007 Vol. 50 pp. 207-215
209
silva2007brazilianimprovement

Abstract

The effect of glucose on xylose-to-xylitol bioconversion by Candida guilliermondii was examined by adding it to sugarcane bagasse hydrolysate medium to obtain different glucose:xylose ratios (1:25, 1:12, 1:5 and 1:2.5). Under experimental conditions, increasing glucose:xylose ratio improved the assimilation of the xylose present in the hydrolysate by yeast, resulting in biomass increase, and in the formation of xylitol and glycerol/ethanol by-products. Maximum values of xylitol yield (0.59 g g-1) and volumetric productivity (0.53 g l-1.h-1) were obtained with glucose:xylose ratio of 1:5, resulting in the higher conversion efficiency (64.3%).
O efeito da glicose na bioconversão de xilose em xilitol por Candida guilliermondii foi avaliado em hidrolisado hemicelulósico de bagaço de cana com diferentes relações glicose:xilose (1:25, 1:12, 1:5 and 1:2,5). Sob as condições experimentais, o aumento da relação glicose:xilose favoreceu a assimilação da xilose presente no hidrolisado, resultando em aumento da biomassa celular e aumento da formação de xilitol e dos sub-produtos glicerol e etanol. Os valores máximos do fator de conversão de xilose em xilitol (0,59 g g-1) e da produtividade volumétrica de xilitol (0,53 g l-1.h-1) foram obtidos com a relação glicose:xilose 1:5, resultando na maior eficiência de conversão (64,3%).

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