cytostatic activity of geranium robertianum l. extracts processed by membrane procedures

cytostatic activity of geranium robertianum l. extracts processed by membrane procedures

;Elena Neagu;Gabriela Paun;Daniel Constantin;Gabriel Lucian Radu
Behavioural brain research 2017 Vol. 10 pp. S2547-S2553
143
neagu2017arabiancytostatic

Abstract

In the present study the antioxidant and cytostatic capacities of some 8% Geranium robertianum (geranium) aqueous extracts processed by membrane procedures (micro- and ultrafiltration) were determined. The extracts were purified by microfiltration and then concentrated by successive ultrafiltrations using Millipore membranes with 10,000 and 1000 Da cut-off. Two methods were used to establish the extracts’ antioxidant activity: DPPH and ABTS; the cytostatic capacity was evaluated on HEp-2p cell lines, by a qualitative method (cytochemical stain with Giemsa solution) and quantitative one (MTT test). Four concentrations of Geranium aqueous extracts were used to test cell viability: 100, 500, 1000 and 2000 μg/mL, 24 and 48 h, against the control culture (100% viability). The concentrated samples had the highest content of total polyphenols and the strongest antioxidant effect, having also the biggest impact upon cell viability.

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0x95644003c57E6F55A65596E3D9Eac6813e3566dA
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238109
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10.1016/j.arabjc.2013.09.028
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