To investigate the effect of biofertilizers and N levels on yield and yield components of wheat, a two-year study (2012-2013) was carried out in Isfahan by using strip split plot based on randomized complete block design in three replications. Four treatments of biofertilizers (control, Nitroxin, Supernitroplus, and Vacosium seed plus) were assigned as vertical factor and four N levels (0, 25, 50 and 100 kg ha-1) were assigned as horizontal factor. Effect of biofertilizer and nitrogen fertilizer on yield and its components as well as the interaction was significant. Use of Nitroxin, supernitroplus, and Vacosium seed plus biofertilizers increased the seed yield by 17, 30 and 11.2 percent, respectively, compared to control treatment. Application of nitrogen at 100 kg ha-1 increased the seed yield by 8, 32 and 38.4 percent, respectively, compared to 50 and 25 kg N ha-1 and control treatments. Use of supernitroplus biofertilizers along with 50 kg N ha-1 produced a seed yield of 6470 kg ha-1 and a harvest index of 42.5 percent, and it was recognized as the best treatment in this study. In summary, the results of this study showed that using a combination of bio-fertilizers and inorganic nitrogen fertilizer can reduce the use of chemical fertilizers and is, therefore, consistent with the goals of sustainable agriculture.