Pyrethrum tatsienense (Bur. et Franch.) Ling is mainly used to treat hepatitis, headaches, head injuries, ulcers, and wounds in Tibet. The aim of this study was to investigate the antioxidant activity of eleven flavonoids mainly revealing luteolin as mother nucleus isolated from Pyrethrum tatsienense, and further elaborate the structure-activity relationships of antioxidant activity about these flavonoids. The above study would provide some theoretical basis for the obvious therapeutic effects of this Chinese medicine. [J Intercult Ethnopharmacol 2014; 3(3.000): 123-127]