In the title compound, C14H18N2O5S, the C—S bond lengths are unequal, with S—Cglucose = 1.8016 (15) Å and S—Cpyridyl = 1.7723 (13) Å. The hydrophilic glucose residues lie in the regions z ≃ 0.25 and 0.75. Four classical hydrogen bonds link the molecules to form layers parallel to the ab plane, from which the pyridyl rings project; pyridyl ring stacking parallel to the a axis links adjacent layers.