In this study we present the biosynthesis of carotenoid pigments and total lipids from the seabuckthorn \"fruit\" and \"seeds\" harvested from the spontaneous flora in 2009 year. The amount of total lipids was determined by the gravimetric method and the content of carotenoids by the spectrophotometric method. After the analysis performed on the biotypes of seabuckthorn collected spontaneously, the carotenoid pigments values are according with the data from literature. The total lipids content is different depending on the nature of the analyzed material and the abiotic conditions of the ecosystem from which plants were harvested.