Cell proliferation and migration on phosphonated polymer surfaces may impair cell adherence and migration. The aim of the present study was to evaluate the ability of osteoblast-like cells to migrate at the surface of the phosphonated chitosan pellicle compared to normal chitosan pellicle. Results. Cells proliferated and migrated at different rates according to surface charge. Negative surfaces showed lower adherence and the migration difference could be quantified. Conclusions. Cell migration on polymer surfaces can be successfully evaluated by improved scratch test performed directly on polymer pellicles. Migration assay can be quantified by measuring the scratch size following separator removal. At the same time, viability assays are applicable following this improved scratch test while with no cells are damaged by mechanical means mechanically damage.