The work was oriented to identification of SCD gene polymorphism in population of Slovak pinzgau cattle. The material involved 91 cows of pinzgau breed kept in Slovakia. Bovine genomic DNA was isolated by fenolchlorophorm deproteinization and ethanol precipitation. The polymorphism of SCD gene was detected by PCR-SSCP method. For the checking of correct genotyping was used PCR-RFLP method. The PCR products were digested with SatI restriction enzyme. The mutant allele C (Ala) was detected by 205bp, 70 bp and 40 bp fragments and the wild allele T (Val)was detected by 205 bp and 117 bp fragments on 3 % agarose gel. In the total population included in the study was detected homozygous genotype CC ( 0.31 ), heterozygous genotype CT (0.42) and homozygous genotype TT( 0.27 ). The frequency of occurrence both alleles proved gently of predominance of mutant allele C – 0.52 before wild allele T – 0.48 .