The study evaluated the effect of yeast supplementation on the digestibility and intake of nutrients of Mangalarga Marchador horses in training. Fourteen Mangalarga Marchador mares were divided into two groups: Probiotic (horses supplemented with 20 g of Saccharomyces cerevisiae daily) and Control. The diet consisted of commercial concentrate and roughage in the ratio of 50:50. The mares were trained for six weeks, Monday to Saturday, and the exercise performed daily alternating work on a treadmill and automatic walker. Nutrient digestibility was assessed using the indicator LIPE® (6 days) end fecal collection was performed for five days. Was analyzed DM, NDF, ADF, CP, GE, hemicelluloses and dry matter intake. There was not difference (P>0,05) in any of the variables analyzed. Supplementation with 20 g of Saccharomyces cerevisiae does not affect the digestibility and nutrient intake in mares Mangalarga Marchador submitted six weeks of aerobic training.