hemagglutination test for the diagnosis of human neurocysticercosis: development of a stable reagent using homologous and heterologous antigens

hemagglutination test for the diagnosis of human neurocysticercosis: development of a stable reagent using homologous and heterologous antigens

;Andréia P. FERREIRA;Adelaide J. VAZ;Paulo M. NAKAMURA;Álvaro T. SASAKI;Antonio W. FERREIRA;José A. LIVRAMENTO
2017 12th international conference for internet technology and secured transactions, icitst 2017 1997 Vol. 39 pp. 29-
202
ferreira1997revistahemagglutination

Abstract

A hemagglutination (HA) test was standardized using formalin- and tannin-treated gander red blood cells sensitized with a total salt extract of C. cellulosae (HA-Cc) and an antigenic extract of Cysticercus longicollis (HA-Cl) vesicular fluid. A total of 61 cerebrospinal fluid (CSF) samples were assayed, 41 from patients with neurocysticercosis and 20 from a control group, which were, respectively, reactive and non-reactive to ELISA using C. cellulosae. The CSF samples from the control group did not react and 35 (85.4%) and 34 (82.9%) CSF samples from patients were reactive to the HA-Cc and HA-Cl tests, respectively. The reagents ready for use were stable up to 6 months when stored at 4°C in 50% glycerol. The present results confirm that the reagent using Cysticercus longicollis stabilized with glycerol can be used as an alternative in the immunological diagnosis of neurocysticercosis
Foi padronizado o teste de hemaglutinação (HA) utilizando hemácias formolizadas e taninizadas de ganso sensibilizadas com extrato salino total de C. cellulosae (HA-Cc) e líquido vesicular de Cysticercus longicollis (HA-Cl). Foram ensaiadas 61 amostras de líquido cefalorraquiano (LCR), 41 de pacientes com neurocisticercose e 20 de um grupo controle, respectivamente, reagentes e não-reagentes no teste ELISA utilizando antígenos de C. cellulosae. As amostras do grupo controle foram não-reagentes e 35 (85,4%) e 34 (82,9%) LCR dos pacientes foram reativos nos testes HA-Cc e HA-Cl, respectivamente. O reagente de hemácias sensibilizadas prontas para uso, foi estável por até 6 meses quando armazenado a 4°C em solução estabilizante com 50% de glicerol. Os resultados confirmam que o reagente de HA-Cl pode ser empregado como alternativa no diagnóstico imunológico da neurocisticercose

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