Objective: The aim of this study was to investigate the influence of intracanal medication on the bond strength of root canal sealer to dentin. Methods: The root canals of 24 bovine incisor were prepared and randomly divided into 4 groups as follows: G1, (control group) no intracanal medication; G2, calcium hydroxide (Ca(OH)2) + chlorhexidine; G3, cotton with Formocresol; and, G4, cotton with camphorated paramonochlorophenol. The samples were stored at temperature of 37 degrees and 100% humidity for 1 week. After, they were sealed with AH Plus sealer and gutta-percha. All roots were sectioned transversely, and the push-out test was performed. The bond strength means were analyzed by analysis of variance. Results: The mean values for the bond strength test and standard deviations were: G1, 2.42 (±1.45); G2, 1.81 (±1.04); G3, 2.21 (±1.14); e, G4, 2.63 (±1.32). The results showed no statistically significant difference between the groups. Conclusion: It can be concluded that intracanal medications used in this study did not influence on the bond strength of AH Plus root canal sealer to dentin.