Taxi trajectories reflect human mobility over the urban roads’ network. Although taxi drivers cruise the same city streets, there is an observed variation in their daily profit. To reveal the reasons behind this issue, this study introduces a novel approach for investigating and understanding the impact of human mobility patterns (taxi drivers’ behavior) on daily drivers’ profit. Firstly, a K-means clustering method is adopted to group taxi drivers into three profitability groups according to their driving duration, driving distance and income. Secondly, the cruising trips and stopping spots for each profitability group are extracted. Thirdly, a comparison among the profitability groups in terms of spatial and temporal patterns on cruising trips and stopping spots is carried out. The comparison applied various methods including the mash map matching method and DBSCAN clustering method. Finally, an overall analysis of the results is discussed in detail. The results show that there is a significant relationship between human mobility patterns and taxi drivers’ profitability. High profitability drivers based on their experience earn more compared to other driver groups, as they know which places are more active to cruise and to stop and at what times. This study provides suggestions and insights for taxi companies and taxi drivers in order to increase their daily income and to enhance the efficiency of the taxi industry.