As a profession, acting is marked by a high-level of economic and social riskiness concomitantly with the possibility for artistic satisfaction and/or public admiration. Current understanding of the psychological attributes that distinguish professional actors is incomplete. Here, we compare samples of professional actors (n = 104), undergraduate student actors (n = 100), and non-acting adults (n = 92) on 26 psychological dimensions and use machine-learning methods to classify participants based on these attributes. Nearly all of the attributes measured here displayed significant univariate mean differences across the three groups, with the strongest effect sizes being on Creative Activities, Openness, and Extraversion. A cross-validated Least Absolute Shrinkage and Selection Operator (LASSO) classification model was capable of identifying actors (either professional or student) from non-actors with a 92% accuracy and was able to sort professional from student actors with a 96% accuracy when age was included in the model, and a 68% accuracy with only psychological attributes included. In these LASSO models, actors in general were distinguished by high levels of Openness, Assertiveness, and Elaboration, but professional actors were specifically marked by high levels of Originality, Volatility, and Literary Activities.