Assembly sequence planning (ASP) of remote handling maintenance in radioactive environment is a combinatorial optimization problem. This study proposes an improved shuffled frog leaping algorithm (SFLA) for the combinatorial optimization problem of ASP. An ASP experiment is conducted to verify the feasibility and stability of the improved SFLA. Simultaneously, the improved SFLA is compared with SFLA, genetic algorithm, particle swarm optimization, and adaptive mutation particle swarm optimization in terms of efficiency and capability of locating the best global assembly sequence. Experiment results show that the proposed algorithm exhibits outstanding performance in solving the ASP problem. The application of the proposed algorithm should increase the level of ASP in a radioactive environment.