Objectives The aim of the study was to determine the effect of mindfulness program on the caregivers’ strain on elderly patients with Alzheimer’s disease who were referred to the Iran Alzheimer’s Association 2012.  Methods & Materials This study was a quasi-experimental study with two groups and before and after design. The participants (n=60) were selected by convenience sampling method and then randomly allocated to intervention (n=30) and control (n=30) groups. The intervention group was trained with a mindfulness program for 6 weeks in two sessions (2 hours in each session per week). Data were collected through the caregiver’s strain questionnaires before and one week after the intervention. Results The mean of caregivers’ strain in the intervention group decreased from 36.96±6.72 to 30.63±6.37 (P<0.05) while in the control group, the mean of caregivers’ strain before (16.7±56.35) and after (86.6±35.80) the study did not show any significant differences (P>0.05). Conclusion The results showed that the mindfulness program could reduce the caregivers’ strain on Alzheimer’s disease, and these findings can be applicable for all caregivers.