The Grain to Green Program (GTGP) and eco-environmental emigration have been employed to alleviate poverty and control rocky desertification in the Southwest China Karst region. Carbon sequestration and oxygen production (CSOP) is used to indicate major ecological changes, because they involve complex processes of material circulation and energy flow. Using remote sensing images and weather records, the spatiotemporal variation of CSOP was analyzed in a typical karst region of northwest Guangxi, China, during 2000–2010 to determine the effects of the Chinese government’s ecological rehabilitation initiatives implemented in 1999. An increase with substantial annual change and a significant increase (20.94%, p < 0.05) in variation were found from 2000 to 2010. CSOP had a highly clustered distribution in 2010 and was correlated with precipitation and temperature (9.18% and 8.96%, respectively, p < 0.05). CSOP was significantly suppressed by human activities (p < 0.01, r = −0.102) but was consistent with the intensity of GTGP (43.80% positive). The power spectrum of CSOP was consistent with that of the gross domestic product. These results indicate that ecological services were improved by rocky desertification control in a typical karst region. The results may provide information to evaluate the efficiency of ecological reconstruction projects.