The effectiveness of clinical parameters in the evaluation of Trypanosoma cruzi infection was analyzed in male Swiss mice at 8 weeks old Animals were divided into HG (healthy) and IG (1400 trypomastigotes, intraperitoneally, Y strain - Trypanosoma cruzi). Quantitative and qualitative parameters were evaluated in non-consecutive days in the period, from 7th-11th and 15th-18th days of infection. There were significant differences (P<0.05) between both groups in both periods regarding water consumption, abdominal circumference and weight. The second group presented differences in amount of excreta, body temperature, move-up and mortality. There was no difference (P>0.05) between the groups in food consumption, exploration of self-cleaning and skin staining. The fecal feature differed between the groups in the second period. The occurrence of isolation was not practical. Differences were observed in the hair between groups, although the parameter had been interfered by fights between animals. The consumption of water, feed, excreta production, characteristic of the faeces, body temperature, abdominal circumference, move up, weight and mortality parameters are easy to be measured and effective in clinical differentiation of healthy mice infected with T. cruzi, elected in protocols for clinical study with mice, which is the first work to gather information of qualitative and quantitative clinical parameters evaluated in these animals.