The population of loggerhead shrike (Lanius ludovicianus excubutirudes) in Grassland National Park of Canada (GNPC) has undergone a severe decline due to habitat loss and limitation. Shrike habitat availability is highly impacted by the biophysical characteristics of grassland landscapes. This study was conducted in the west block of GNPC. The overall purpose was to extract important biophysical and topographical variables from both SPOT satellite imagery and in situ measurements. Statistical analysis including Analysis of Variance (ANOVA), measuring Coefficient Variation (CV), and regression analysis were applied to these variables obtained from both imagery and in situ measurement. Vegetation spatial variation and heterogeneity among active, inactive and control nesting sites at 20 m × 20 m, 60 m × 60 m and 100 m × 100 m scales were investigated. Results indicated that shrikes prefer to nest in open areas with scattered shrubs, particularly thick or thorny species of smaller size, to discourage mammalian predators. The most important topographical characteristic is that active sites are located far away from roads at higher elevation. Vegetation index was identified as a good indicator of vegetation characteristics for shrike habitats due to its significant relation to most relevant biophysical factors. Spatial variation analysis showed that at all spatial scales, active sites have the lowest vegetation abundance and the highest heterogeneity among the three types of nesting sites. For all shrike habitat types, vegetation abundance decreases with increasing spatial scales while habitat heterogeneity increases with increasing spatial scales. This research also indicated that suitable shrike habitat for GNPC can be mapped using a logistical model with ATSAVI and dead material in shrub canopy as the independent variables.