The goal of this study was to determine the feasibility of culturing high-oil algae biomass based on wastewater from dairy processing plants. The experiments were conducted in laboratory scale with tubular photobioreactor using. The best technological properties were demonstrated for eluates from an anaerobic reactor treating dairy wastewater. The use of a substrate of this type yielded algae biomass concentration at a level of 3490 mg d.m./dm³, with the mean rate of algae biomass growth at 176 mg d.m./dm³∙d. The mean content of oil in the proliferated biomass of algae approximated 20%.