Carbon Spheres were fabricated by hydrothermal method and their structural properties were investigated. Carbon Spheres with average sizes around of 230, 320 and 430 nm were synthesized in different concentration of glucose aqueous solution and different hydrothermal reaction time. The temperature of 180 °C by a hydrothermal reaction was fixed in all of glucose concentration and hydrothermal reaction time. The result showed that the spherical shape of Carbon Spheres was formed in the special concentration of glucose aqueous solution and hydrothermal reaction time.The product obtained was characterized by X-ray diffraction(XRD); Fourier transforms infrared (FTIR) spectra, which have inferred the Carbonic nature of the product. Further,SEM images have revealed the spheres having quite spherical morphology.