Most of the grain legumes in Pakistan are poorly nodulated either because of low indigenous rhizobialpopulation or due to inefficient strains of native rhizobia. However, improvement in nodulation could be achievedthrough inoculation with effective rhizobial strains. A pot trial was conducted to evaluate the effect of Rhizobiuminoculation along with L-tryptophan (L-TRP), a precursor of indole acetic acid (IAA) on growth, yield andnodulation of mash been. The results indicated that Rhizobium inoculation along with L-tryptophan application (6mg kg-1 soil) significantly improved plant height (44.6%) as compared to control. Different improvements in rootlength (72.4%), oven dry root weight (Five fold), no. of pods plant-1 (86.4%), no. of grains per pod (42.8%), 100-grain weight (18.9%) and nitrogen concentration in grains (two fold), was noted as compared with the controlwhere L-TRP was applied @ 2 mg kg-1 soil. There was a significant increase in number of nodules plant-1 (one fold),nodule fresh weight plant-1 (two fold) and nodule dry weight plant-1 (four fold) with Rhizobium inoculation alongwith L-TRP application @ 2 mg kg-1 soil as compared to Rhizobium inoculation alone. The study showed thatRhizobium inoculation along with L-TRP application could be a better approach for sustainable legume production.