Preeclampsia (PE) is one of the main causes of fetal and maternal mortality. The analysis of candidate gene polymorphisms can improve our understanding of the mechanisms underlying pathogenesis of PE. Present study is aimed at investigating the association between c.66A > G, c.677C > T, c.1298A > C, and c.2756A > G polymorphisms and PE in Iranian women. About 117 women with history of PE and 103 healthy women with a pregnancy not complicated by PE were selected. The genomic DNA was extracted from peripheral blood. Single-nucleotide polymorphisms were genotyped using Real-Time PCR. There was a significant difference between c.677C > T polymorphism with PE ( = 0.045). The frequency of C/T heterozygous genotypes were (58% vs. 36%) in the case and control groups, respectively. There were no statistically significant differences between other genetic polymorphisms. The results indicated that the c.677C > T polymorphism may be associated with development of PE in Iranian women.