Alien plants recorded in the Kruger National Park are listed, providing an update of species that have been recorded since the last published list in 1988. The serious consequences that invasive alien plants pose are widely recognised internationally and are regarded as the greatest threat to the Kruger National Park. It is extremely important to maintain an updated list of species to use as a reference and from which to monitor changes in the influx of invasive species. The list contains the invasive status of the plants, current impact, habitat types, biological control status and status according to the Conservation of Agricultural Resources Act (CARA), Act 43 of 1983, as amended in March 2001. The list comprises 370 species, of which 121 are invaders and two are transformer weeds, which may cause considerable damage and alterations to the indigenous biodiversity. A total of 91 species listed herein are also listed in the CARA regulations or are proposed additions to the CARA regulations.