An indexing of the organs (radicle and plumule) and components (husk, endosperm and embryo) of rice seeds using Enzyme Linked Immunosorbent Assay (ELISA) was carried out to detect Rice yellow mottle virus (RYMV) and establish the exact location of the virus in the rice seed. RYMV was detected only in the husk (seed coat) but not in the endosperm, plumule, radicle, nor embryo. None of the seedlings raised from the seeds expressed RYMV symptoms. No virus particle was detected by the ELISA test in the leaves of the screenhouse-reared plants obtained from seeds of infected plants. The results indicate that RYMV is apparently not transmitted through rice seed probably because the virus is seed-borne in the husk (seed coat) of mature rice seeds.