Background: Hatha Yoga (HY) can be an alternative to improve physical activity in middle- aged and older women. However, conventional HY (CHY) exercising may not result in enough training stimulus to improve cardiovascular fitness. The purpose of this study was to evaluate the effect of an intensive HY intervention (IHY) on cardiovascular risk factors in middle-aged and older women from Northern Mexico. Materials and Methods: In this prospective quasiexperimental design, four middle-aged and nine older CHY practicing females (yoginis) were enrolled into an 11-week IHY program consisting of 5 sessions/week for 90 min (55 sessions). The program adherence, asana performance, and work intensity were assessed along the intervention. Anthropometric [body mass index (BMI), % body fat and ∑ skin folds], cardiovascular fitness [maximal expired air volume (VE _max ), maximal O ₂ consumption (VO _2max ), maximal heart rate (HR _max ), systolic (BPs) and diastolic blood pressure (BPd)], biochemical [glucose, triacylglycerols (TAG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C)], and dietary parameters were evaluated before and after IHY. Results: Daily caloric intake (~1,916 kcal/day), program adherence (~85%), and exercising skills (asana performance) were similar in both middle-aged and older women. The IHY program did not modify any anthropometric measurements. However, it increased VO _2max and VE _max and HDL-C while TAG and LDL-C remained stable in both middle-aged and older groups (P < 0.01). Conclusions: The proposed IHY program improves different cardiovascular risk factors (namely VO _2max and HDL-C) in middle-aged and older women.