We apply the principles of atmospheric surface layer dynamics within a vineyard canopy to demonstrate the use of forward-looking infrared cameras measuring surface brightness temperature (spectrum bandwidth of 7.5 to 14 μm) at a relatively high temporal rate of 10 s. The temporal surface brightness signal over a few hours of the stable nighttime boundary layer, intermittently interrupted by periods of turbulent heat flux surges, was shown to be related to the observed meteorological measurements by an in situ eddy-covariance system, and reflected the above-canopy wind variability. The infrared raster images were collected and the resultant self-organized spatial cluster provided the meteorological context when compared to in situ data. The spatial brightness temperature pattern was explained in terms of the presence or absence of nighttime cloud cover and down-welling of long-wave radiation and the canopy turbulent heat flux. Time sequential thermography as demonstrated in this research provides positive evidence behind the application of thermal infrared cameras in the domain of micrometeorology, and to enhance our spatial understanding of turbulent eddy interactions with the surface.