The usage of Epilobium angustifolium L. in folk medicine against prostate and skin diseases encouraged us to investigate pBR322 DNA-binding ability, biological activities and chemical composition of wild growing Epilobium angustifolium L.’s methanolic, ethanolic and aqueous extracts from Canakkale, Turkey. DNA-binding activity was studied by agarose gel electrophoresis and chemical composition via GC, GC-MS, HPLC, LC-MS, ICP-MS and AAS. Literature survey indicates no data on pBR322 plasmid DNA interaction of Epilobium species which is an important criteria for being a potential anticancer agent. Antibacterial and antifungal effects were tested against 11 bacterias and 3 fungis and compared with ampicillin, chloramphenicol and ketokanozol by agar well diffusion techniques. Total phenolic content was found by Folin-Ciocalteu method and antioxidant activity was obtained by DPPH and Fe (II)-FZ (ferric-ferrozine) methods. This plant is rich of flavonoids, hormones, fatty and phenolic acids. Si, K, Mg, P and Mn were the main inorganic elements. Epilobium angustifolium L. with its high DNA-binding activity and rich chemical composition can be a good potential anticancer, antimicrobial and antioxidant prodrug.