The paper compares the results of an application of three selected systems (TRANSIMS, SUMO, and VISSIM) to the microscopic simulation of traffic flow for a fragment of a real urban road network. First, the problem of traffic flow modeling and simulation was described, and the selected systems were introduced. Afterwards, model construction and simulation were presented. The authors discussed an issue of model calibration, and then conducted a comparative result analysis for the three systems, with a reference to the real traffic. The paper ends with a summary of the conducted research.