detection of protozoans babesia microti and [i]toxoplasma gondii[/i] and their co-existence in ticks (acari: ixodida) collected in tarnogórski district (upper silesia, poland)

detection of protozoans babesia microti and [i]toxoplasma gondii[/i] and their co-existence in ticks (acari: ixodida) collected in tarnogórski district (upper silesia, poland)

;Marek Asman;Krzysztof Solarz;Piotr Cuber;Tomasz Gąsior;Piotr Szilman;Ewa Szilman;Ewelina Tondaś;Anna Matzullok;Natalia Kusion;Karol Florek
biomedicine and pharmacotherapy 2015 Vol. 22 pp. 80-83
226
asman2015annalsdetection

Abstract

[b]Introduction[/b]. Ticks (Acari: Ixodida) are vectors of many pathogens i. a: [i]Babesia microti [/i][Bm] and [i]Toxoplasma gondii[/i] [Tg]. In Poland, Ixodes ricinus [Ir] is the main vector of both pathogens. This tick species and pathogens transmitted by them are a significant threat to human and animal health. objectives of the study. Detection of the protozoans Bm and Tg in ticks collected in the Tarnogórski district area. [b]Material and methods[/b]. The ticks were collected from vegetation and pets in the spring period of their activity on the territory of the Tarnogórski district. The parasites were preserved in 70% ethanol. DNA was isolated by ammonia method. Bm was detected by nested-PCR using specific primers for the 18S rRNA sequence. To detect the Tg a commercial kit was used. The PCR products were separated on 2% ethidium bromide stained agarose gels and visualised under UV light. [b]Results[/b]. It was showed that all collected ticks belonged to the species Ir. Bm was detected in 50.87% and Tg in 64.91% of all examined ticks. Co-existence of these both protozoans in 36.84% of total examined ticks was noted. [b]Conclusions[/b]. The study showed a high risk of exposure to Bm and Tg in the studied area. Ticks Ir play an important role in the transmission of Bm in this region. Demonstrating a high percentage of ticks collected from animals infected with Tg may indicate their important role in the transmission of this pathogen, but it requires a further studies.

Citation

ID: 152208
Ref Key: asman2015annalsdetection
Use this key to autocite in SciMatic or Thesis Manager

References

Blockchain Verification

Account:
NFT Contract Address:
0x95644003c57E6F55A65596E3D9Eac6813e3566dA
Article ID:
152208
Unique Identifier:
Network:
Scimatic Chain (ID: 481)
Loading...
Blockchain Readiness Checklist
Authors
Abstract
Journal Name
Year
Title
5/5
Creates 1,000,000 NFT tokens for this article
Token Features:
  • ERC-1155 Standard NFT
  • 1 Million Supply per Article
  • Transferable via MetaMask
  • Permanent Blockchain Record
Blockchain QR Code
Scan with Saymatik Web3.0 Wallet

Saymatik Web3.0 Wallet