Physic nut is a plant widely cultivated in various regions of tropical America, Asia and Africa that adapts well to arid and degraded soils. This essay aimed to evaluate the effect of arbuscular mycorrhizal fungi (AMF) on solarized substrate for production of seedlings with seeds from states of Pernambuco, Minas Gerais and Mato Grosso do Sul, Brazil. AMF used included a mixture of Clarum glomus, Gigaspora margarita, Scutellospora hterogama and Gigaspora calospora. Treatments consisted of inoculation in that mixture with solarized substrate, non-solarized and control (without inoculation). The results showed that seedlings produced with seeds from Pernambuco had the lowest values for leaf number and the ratio between height and diameter, while those formed with seeds from Mato Grosso do Sul revealed the largest height and diameter. Inoculation with AMF in solarized oxisol red (LV) substrate did not alter morphology of physic nut seedlings produced from seeds of the three provenances tested.