The Eschweilera genus has great ecological and economic importance due to its wide abundance in the Amazon basin. One potential use for the Eschweilera genus is in forest management, where just a few trees are removed per hectare. In order to improve the forest management in the Amazon, this study assessed two critical issues: volume equations fitted for a single genus and the development of a non-destructive method using climbing techniques. The equipment used to measure the sample trees included: climbing rope, ascenders, descenders, and carabiners. To carry out the objectives of this study, 64 trees with diameter at breast height (DBH) ≥ 10 cm were selected and measured in ZF-2 Tropical Forestry Station near the city of Manaus, Brazil. Four single input models with DBH and four dual input models with DBH and merchantable height (H) were tested. The Husch model (V = a × DBHb) presented the best performance (R2 = 0.97). This model does not require the merchantable height, which is an important advantage, because of the difficulty in measuring this variable in tropical forests. When the merchantable height data are collected using accurate methods, the Schumacher and Hall model (V = a × DBHb × Hc) is the most appropriated. Tree climbing techniques with the use of ropes, as a non-destructive method, is a good alternative to measure the merchantable height, the diameter along the stem, and also estimate the tree volume (m3) of the Eschweilera genus in the Amazon basin.