Transparent sol-gel film with antibacterial coating property incorporating silver nanoparticles has been successfully developed. Silver nanoparticles were synthesized by precipitation method at room temperature. XRD structural studies show that crystallite sizes in the range of 18 nm to 40 nm were produced. The coating system used methyltrimethoxy silane as binder and N-propanol as diluent to obtain the highest transperancy. 2.5% wt of nanosilver crystallites was added as antibacterial agent. The coating mixture was applied onto glass plates using sponges and tested against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Values of antimicrobial activity of 4.6, 7.2, and 4.2 were, respectively, obtained for Staphylococcus aureus, Escherichia coli and Pseudomonas Aeruginosa. Coating with antimicrobial activity greater than 2 classified as antibacterial.