Probiotics are live microorganisms that, when administered in an adequate amount, confer a health benefit on the host through the gut. is a widespread yeast found in nature. This microorganism has been used as a probiotic agent in recent years. In this study, the effect of microencapsulation on survival rate of in the simulated gastrointestinal tract medium and the impact of microencapsulated on some serum biochemical factors in a rat model was evaluated.30 male wistar rats were divided into three groups (control, rats receiving microencapsulated , and rats receiving alone). The probiotic was gavaged at a dosage of 2 gr/kg BW for 8 weeks. Blood was collected from rats at the end of the treatment period and biochemical factors were measured using Mancompany kits.The results showed a significant increase in viability of microencapsulated in comparison with free (p<0.05). Weight of rats in probiotic treated groups was significantly higher in comparison with the control group (p<0.05). Moreover, probiotic treatment reduced mean levels of triglycerides, cholesterol, free blood sugar and liver enzymes in rats.Microencapsulation could increase the survival rate of yeast probiotics in the gastrointestinal tract; however, more studies are needed for better understanding of the exact effect of microencapsulation on probiotics' function.