Genome sequencing in microfabricated high-density picolitre reactors
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Genome sequencing in microfabricated high-density picolitre reactors

Margulies M;Egholm M;Altman WE;Attiya S;Bader JS;Bemben LA;Berka J;Braverman MS;Chen YJ;Chen Z;Dewell SB;Du L;Fierro JM;Gomes XV;Godwin BC;He W;Helgesen S;Ho CH;Irzyk GP;Jando SC;Alenquer ML;Jarvie TP;Jirage KB;Kim JB;Knight JR;Lanza JR;Leamon JH;Lefkowitz SM;Lei M;Li J;Lohman KL;Lu H;Makhijani VB;McDade KE;McKenna MP;Myers EW;Nickerson E;Nobile JR;Plant R;Puc BP;Ronan MT;Roth GT;Sarkis GJ;Simons JF;Simpson JW;Srinivasan M;Tartaro KR;Tomasz A;Vogt KA;Volkmer GA;Wang SH;Wang Y;Weiner MP;Yu P;Begley RF;Rothberg JM;;
Nature 2005 Vol. 437 pp. -
329
m2005naturegenome

Abstract

The proliferation of large-scale DNA-sequencing projects in recent years has driven a search for alternative methods to reduce time and cost. Here we describe a scalable, highly parallel sequencing system with raw throughput significantly greater than that of state-of-the-art capillary electrophores …

Keywords

National Center for Biotechnology Information NCBI NLM MEDLINE pubmed abstract nih national institutes of health national library of medicine reproducibility of results research support u.s. gov't N.I.H. Extramural P.H.S. Sequence Analysis genome polymerase chain reaction sensitivity and specificity bacterial* time factors electrophoresis genomics / instrumentation* pmid:16056220 pmc1464427 doi:10.1038/nature03959 marcel margulies michael egholm jonathan m rothberg capillary emulsions fiber optic technology genomics / economics microchemistry / economics microchemistry / instrumentation* mycoplasma genitalium / genetics* dna / economics dna / instrumentation*

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http://www.ncbi.nlm.nih.gov/pubmed/16056220

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