AbstractThis article concerns the widespread matter of biophysical comfort. In this work, 10 double-layer knitted fabrics with potential application in multilayer garments addressed to a specific group of users, such as newborns, were investigated. The materials were constructed with the following raw materials: cotton, polypropylene, polyester, polyamide, bamboo, and viscose. The textiles with a comparable geometrical structure and different composition were tested for their air permeability. In the experimental part, the materials were tested in specific constant ambient conditions using an air permeability tester. In the simulation part, 3D models of actual textiles were designed and air permeability based on the performed simulations using finite volume method was calculated. Both measurements and simulations yielded comparable results and showed that the air permeability of the knitted fabric strongly depends on the thickness and geometrical parameters of yarn.