Single point quantification of antibody by ELISA without need of a reference curve

Single point quantification of antibody by ELISA without need of a reference curve

Dopatka HD;Giesendorf B;;
journal of clinical laboratory analysis 1992 Vol. 6 pp. -
320
hd1992journalsingle

Abstract

A new method for the quantification of antibodies in the enzyme-linked immunosorbent assay is described. This procedure replaces titer determinations based on end-point dilution of the antibody under investigation. Here, the sample is tested in a single dilution and the optical density (OD) obtained …

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