Non-contact detection and estimation of vital signs such as respiratory and cardiac frequencies is a powerful tool for surveillance applications. In particular, the continuous wave bio-radar has been widely investigated to determine the physiological parameters in a non-contact manner. Since the RF-reflected signal from the human body is corrupted by noise and random body movements, traditional Fourier analysis fails to detect the heart and breathing frequencies. In this effort, cyclostationary analysis has been used to improve the radar performance for non-invasive measurement of respiratory rate and heart rate. However, the preliminary works focus only on one frequency and do not include the impact of attenuation and random movement of the body in the analysis. Hence in this paper, we evaluate the impact of distance and noise on the cyclic features of the reflected signal. Furthermore, we explore the assessment of second order cyclostationary signal processing performance by developing the cyclic mean, the conjugate cyclic autocorrelation and the cyclic cumulant. In addition, the analysis is carried out using a reduced number of samples to reduce the response time. Implementation of the cyclostationary technique using a bi-static radar configuration at 2.5 GHz is shown as an example to demonstrate the proposed approach.