The full-scale static testing of wind turbine blades is an effective means to verify the accuracy and rationality of the blade design, and it is an indispensable part in the blade certification process. In the full-scale static experiments, the strain of the wind turbine blade is related to the applied loads, loading positions, stiffness, deflection, and other factors. At present, researches focus on the analysis of blade failure causes, blade load-bearing capacity, and parameter measurement methods in addition to the correlation analysis between the strain and the applied loads primarily. However, they neglect the loading positions and blade displacements. The correlation among the strain and applied loads, loading positions, displacements, etc. is nonlinear; besides that, the number of design variables is numerous, and thus the calculation and prediction of the blade strain are quite complicated and difficult using traditional numerical methods. Moreover, in full-scale static testing, the number of measuring points and strain gauges are limited, so the test data have insufficient significance to the calibration of the blade design. This paper has performed a study on the new strain prediction method by introducing intelligent algorithms. Back propagation neural network (BPNN) improved by Particle Swarm Optimization (PSO) has significant advantages in dealing with non-linear fitting and multi-input parameters. Models based on BPNN improved by PSO (PSO-BPNN) have better robustness and accuracy. Based on the advantages of the neural network in dealing with complex problems, a strain-predictive PSO-BPNN model for full-scale static experiment of a certain wind turbine blade was established. In addition, the strain values for the unmeasured points were predicted. The accuracy of the PSO-BPNN prediction model was verified by comparing with the BPNN model and the simulation test. Both the applicability and usability of strain-predictive neural network models were verified by comparing the prediction results with simulation outcomes. The comparison results show that PSO-BPNN can be utilized to predict the strain of unmeasured points of wind turbine blades during static testing, and this provides more data for characteristic structural parameters calculation.